Genome Sequencing
Prospective Students
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PI: Yasuko Rikihisa
Contact Info:
 Dept. of Veterinary
 The Ohio State Univ.
 1925 Coffey Road
 Columbus, OH 43210
 Phone: 614-292-5661
OSU Homepage
Dept. of Vet. Biosciences
Ehrlichial Genomic and Proteomic Projects
Funded by the National Institutes of Health (NIH) Grant # R01 AI47885
Project Description:
Determine the complete nucleotide sequences of genomes of three divergent human Ehrlichiosis agents: Ehrlichia chaffeensis, Anaplasma phagocytophilum, and Neorickettsia sennetsu.
Comparative analysis of sequenced genomes of Rickettsiales and other bacteria. 
  (For background information of human Ehrlichiosis, agents of Ehrlichiosis, and Ehrlichial Research Laboratory, please click on buttons on left panel to navigate the entire website).
dots Proteomics Expression Analysis from A. phagocytophilum and E. chaffeensis-infected HL-60 cells:
  folder Complete Bacterial Proteomes: A. phagocytophilum and E. chaffeensis Proteins;
  folder MS/Quantitaion Data: A. phagocytophilum and E. chaffeensis Proteins  |   Ratios of Human Proteins in Infected vs. Uninfected HL-60 Cells;
  folder Human Proteins: Uninfected | AP-Infected | EC-Infected | more ...
dots TIGR-CMR: Genome Properties.
bullets TIGR-CMR (Comprehensive Microbial Resource) Homepage .
bullets MIST (Microbial Signal Transduction database): Bacterial One/Two-Component Systems.
NCBI BLAST Search (Basic Local Alignment Search Tool).
BLAST with Microbial Genomes (1354 bacterial/58 archaeal/239 eukaryotic genomes tree, and counting).
NCBI Conserved Domain Database and Search.
Entrez Protein Search in the Entrez search and retrieval system.
Project Progress Status:
  • January 2011: Proteomic analysis of Anaplasma phagocytophilum and Ehrlichia chaffeensis was published at Frontiers in Microbiology (Frontiers in Cellular and Infection Microbiology Section).
  • August 2009: Genome sequence of Neorickettsia risticii was completed and published at Nucleic Acids Research.
  • January 2006: Analysis of the three genomes was completed, and the manuscript is published at PLoS Genetics (Feb 2006 issue).
  • December, 2004: All genome sequences are closed and the final annotation/manual curation/point mutation analysis is in progress.
  • June 3, 2003: Genome sequences of E. chaffeensis and N. sennetsu are completed and analysis is in progress.
  • July 5, 2002: TIGR has completed random sequencing of E. chaffeensis and gap closure is under way (41 contigs; 1,205,083 total letters).
    Also, random sequencing of Anaplasma phagocytophilum is in progress.
Last Updated: February 25, 2011. | Maintained by: Mingqun Lin. | © OSU Rickettsiology Lab, 2001 - 2017.
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